Plant tissue culture

[sneaky snake]

I have a very rare plant that I want to grow but I don't think I'll be able to keep it alive as it is, so I think I need to use a tissue culture and grow it from that. Is there any way to grow it from dried tissue or should I put it in a fridge? What kind of medium would I grow it in? I'm kind of familiar with tissue culture of mushrooms but I don't know much about plants.

[scovegner]

What plant?

[sneaky snake]

Obviously you either don't want to help me or can't since all you did was ask which kind of plant it is. But I'll tell you anyway, it's a salvia divinorum plant with an unusually high rate of seed viability. I've never had good success with salvia d, but these seeds are not only viable, but they also produce a higher than average viability rate than the average salvia d plants. The problem is the rate is still nowhere near the mother plant and I need to back cross it in order to breed for this trait to be dominant.

[sneaky snake]

Also, I have looked it up and read about it but mostly the sites that talk about plant tissue culture either want to sell a kit or don't actually go into the full details. This plant is dying and I need to do something fast. I guess it doesn't matter though. Someone somewhere will eventually come across a similar plant. I just hope they know what they're doing when they do.

[Alamo]

... Im confused, first if its alright with you that is. Camei n asked about how to save your plant... without telling us what kind of plant your trying to save. That's like asking a mechanic "whats wrong with my car, he goes: what car" now its a very basic part in solving your problem.

Anyway hehe, I am assuming you live somewhere were it is illegal and thus your "rare plant" problem.

Robbed first click Google searches...

Salvia Divinorum plants can be propagated through cuttings and they require rich soil. Moisture should be high, but plants can be slowly adjusted to 'normal' growth conditions.

Plants are shipped as tissue culture plantlets still in the tube. This makes it much easier for us to ship them and you will get a 100% disease and pest-free plant.

To transplant your tissue culture Salvia Divinorum to soil you will need a pot, some fine soil and a glass jar. A fungicide such as Thiram is advisable.

The Salvia Divinorum plant is taken out of the tube and the agar is washed off with luke warm water. The roots must be clean! Any dead/black material is removed. Sometimes the tops of the plantlets die from the shock of shipment. This is no problem, just clip off the dead tissue and new shoots will appear from the leaf axils.

The bottom leaves of the Salvia Divinorum plant are removed and the larger leaves are trimmed. Large leaves will lead to too much moisture loss which leads to the wilting of the plantlet.

Carefully plant Salvia Divinorum in the soil and water it (preferably with a fungicide solution). If no fungicide is available, the soil should have been heat treated to prevent infection. A glass jar is put over the plantlet to raise humidity and it is put in a light shady place. After 7 days new growth should be visible and after 14 days the Salvia Divinorum plant can slowly be adjusted normal growth conditions.

In the winter some additional lighting may be necessary.

Once Salvia Divinorum is established it grows pretty fast. The Salvia Divinorum plants should be topped regularly to make them bushier or they will grow tall and thin and fall over.

Salvia cuttings are one of the easiest to propagate with no rooting gel or nothing. like nag said, you can put it into a rock wool thing (i find that pointless n time consuming) but also, they can go straight into most good soil mixes with or without roots and ittle root quickly as long as u keep it in a high humidity area ( easy way to do so is just cut a coke bottle and place over the the clone) till it starts moving, then try to setup a good humid grow area for em. Or you can just chuck an unrooted clone into a cup with abit of water for a week or so till the roots pop out then plant.

I guess the moral of the story, try, then if no luck ask politely.

[sneaky snake]

Salvia divinorum is rare even where it's legal. And it's not illegal here yet but I think it will be eventually. My plant is more rare than the usual salvia d because of the viability of the seeds. My problem with it has nothing to do with what kind of plant it is, so that wasn't necessary to say. It could've been any kind of rare plant and all I'd need to know was still about tissue culture, which type of agar, where to buy the basic stuff, what antibiotics, etc. Basic plant tissue culture stuff. I was wanting help from someone with experience in tissue culture, not specifically salvia divinorum. Thanks for trying to help though.

The plant is infected and won't be alive much longer. I don't have a flowhood or anything like that and I just wanted some info on what type of medium to use. Nevermind though, if I were to order a kit for tissue culture the plant would be dead before it got here.

[Alamo]

Might want look at a murashige-skoog medium for that agar. As for the infected part what exactly is infected, could be a variety of things that can be treated many different ways :).

[T.K. Baha]

Yeah don't get an attitude when you are the one asking for help you little shit. As far as doing a tissue culture you can't with plants like you can mushrooms. For plants you need specific parts to propagate them. Dried plant matter is especially useless other than seeds. Your best bet is going to be to make a cutting from the node which might die and kill the mother plant so its a tricky situation. Why not make a node cutting, dip in some root hormone and hope for the best? Asshole.

And as far as I know all the random jargon you threw out above only applies in mycology.

[sneaky snake]

Yeah don't get an attitude when you are the one asking for help you little shit. As far as doing a tissue culture you can't with plants like you can mushrooms. For plants you need specific parts to propagate them. Dried plant matter is especially useless other than seeds. Your best bet is going to be to make a cutting from the node which might die and kill the mother plant so its a tricky situation. Why not make a node cutting, dip in some root hormone and hope for the best? Asshole.

And as far as I know all the random jargon you threw out above only applies in mycology.
Might want to be careful who you're calling a little shit. I'm most likely a lot bigger and stronger than you, little peon. I didn't get an attitude but you're irritating me now. None of your advice was worth anything. You're an idiot, and I'm not going to read anything you type ever again.

[JREwing]

Really, google aeroponic propogator. They have the best success rate.

[monotoned]

actually SAS is right. You can't culture with dried plant parts, and usually you use the meristems (apical meristem is said to work best though).

As for the medium every plant requires a different combination, but basically it's water, the essential macro and micro elements for plants, sucrose, amino acids, plant hormones, etc. A lot more complicated than the ones you use for fungi.

You can still try, but you're new to plant tissue culture so don't get your hopes up.
Anyways, good luck.

[Klepto]

a cutting would be your best bet. tissue culture actually takes some schooling while any novice gardener can root a cutting. best of luck

[sneaky snake]

I deleted this topic since the plant is dead now and I didn't want anymore asshats bitching at me but apparently someone felt it would be funny to undelete it...

[ShutMeUp]

I deleted this topic since the plant is dead now and I didn't want anymore asshats bitching at me but apparently someone felt it would be funny to undelete it...

Do you want me to delete it again?

[sneaky snake]

Not really any point in redeleting it. Why was it undeleted though?

[T.K. Baha]

Because we like to laugh at your misfortunes. Haha your plant is dead. Maybe if you would have had some manners it would still be alive. Im sure it is slowly simmering in plant hell as we speak. :rofl:

[ShutMeUp]

Not really any point in redeleting it. Why was it undeleted though?

I don't know, I wasn't aware it had been deleted.

[Venom]

Look on the bright side at least you got some advice which was better than the usual stupid search google comments.